Serum levels of microRNA-371a-3p (M371) represent a novel and sensitive biomarker of germ cell tumours (GCTs). This study analysed the utility of M371 to identify viable cancer (VC) in postchemotherapy (pc) residual masses with the underlying goal of avoiding overtreatment.
A multicentric, prospective diagnostic study was conducted in 180 GCT patients undergoing pc resection of residual masses. A correlation of M371 measurement results with the histological presence of VC in masses was found. A receiver operating characteristic analysis was performed for exploring the performance characteristics of the test.
The sensitivity was found to be 68.9%, specificity 99.3%, area under the curve 0.813, positive predictive value 0.969, and negative predictive value 0.905; sensitivity is significantly associated with the percentage of VC in the mass. In specimens with ≤10% VC, there were 33.3% elevated M371 levels as opposed to 85.7% in specimens with >50% VC. Teratoma and somatic-type malignancy do not express M371. A lack of a central pathological review is a limitation.
The M371 test can identify 68.9% of patients with VC in pc masses. However, cases with <10% VC in the mass may escape detection. Teratoma does not express M371. The test alone cannot correctly identify patients requiring pc surgery, but it may be a tool for scheduling the extent of surgery.
The microRNA-371a-3p (M371) test can identify about two-thirds of patients with viable cancer in residual metastatic masses following chemotherapy for germ cell tumours. Only masses with high percentages of viable cancer cells can be identified, and the histological subtype teratoma remains undetected with the test.
European urology focus. 2024 May 09 [Epub ahead of print]
Klaus-Peter Dieckmann, Francesca Grobelny, Armin Soave, Yue Che, Tim Nestler, Cord Matthies, Julia Heinzelbecker, Alexander Winter, Axel Heidenreich, Tabea Niemzok, Cansu Dumlupinar, Markus Angerer, Christian Wülfing, Pia Paffenholz, Gazanfer Belge
Department of Urology, Asklepios Klinik Altona, Hamburg, Germany., Department of Tumour Genetics, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany; Faculty of Medicine, University of Hamburg, Hamburg, Germany., University Medical Center Hamburg-Eppendorf, Department of Urology, Hamburg, Germany., Department of Urology, Heinrich-Heine-University Duesseldorf, Duesseldorf, Germany., Department of Urology, Federal Armed Services Hospital Koblenz, Koblenz, Germany., Department of Urology, Federal Armed Services Hospital, Hamburg, Germany., Department of Urology, Saarland University Medical Centre, Saarland University, Homburg, Germany., Department of Urology, University Hospital Oldenburg, Oldenburg, Germany., Department of Urology, University Hospital of Cologne, Cologne, Germany., Department of Tumour Genetics, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany., Department of Tumour Genetics, Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany. Electronic address: .