Despite the progress made in the early detection and treatment of prostate adenocarcinoma, the metastatic lesions from this tumor are incurable.
We used genome-wide expression analysis of human prostate cancer cells with different metastatic behavior in animal models to reveal that bone-tropic phenotypes up-regulate three genes encoding for the cytokine Interleukin-1beta (IL1B, IL-1β), the chemokine CXCL6 (GCP-2) and the protease inhibitor Elafin (PI3). The Oncomine database revealed that these three genes are significantly upregulated in human prostate cancer versus normal tissue and correlate with Gleason scores ≥7. This correlation was further validated for IL-1β by immuno-detection in prostate tissue arrays. Our study also shows that the exogenous over-expression of IL-1β in non-metastatic cancer cells promotes their growth into large skeletal lesions in mice, whereas its knockdown significantly impairs the bone progression of highly metastatic cells. In addition, IL-1β secreted by metastatic cells induced the over-expression of COX-2 (PTGS2) in human bone mesenchymal cells treated with conditioned media from bone metastatic prostate cancer cells. Finally, we inspected human tissue specimens from skeletal metastases and detected prostate cancer cells positive for both IL-1β and synaptophysin (SYP) while concurrently lacking prostate specific antigen (PSA, KLK3) expression. Collectively, these findings indicate that IL-1β supports the skeletal colonization and metastatic progression of prostate cancer cells with an acquired neuroendocrine phenotype.
Written by:
Liu Q, Russell MR, Shahriari K, Jernigan DL, Lioni MI, Garcia FU, Fatatis A. Are you the author?
Department of Pharmacology & Physiology, Drexel University College of Medicine.
Reference: Cancer Res. 2013 Mar 27. Epub ahead of print.
doi: 10.1158/0008-5472.CAN-12-3970
PubMed Abstract
PMID: 23536554
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