BACKGROUND: Research has shown reduced expression levels of miR-154 in prostate cancer (CaP).
However, the function and molecular mechanisms of miR-154 in this cancer type remains unknown.
OBJECTIVE: The aims of this study were to examine the functional significance of miR-154 in CaP cells and to identify the novel molecular targets regulated by miR-154.
MATERIALS AND METHODS: miR-154 expression significantly decreased in primary CaP samples compared with nonmalignant samples measured by quantitative reverse transcription polymerase chain reaction. Restoration of miR-154 lowered the potential of CaP cell lines to grow and proliferate in vitro evaluated by CCK-8 assay, colony formation assay, and flow cytometry. miR-154 down-regulated the expression of CCND2 by binding to its 3'-untranslated region by luciferase reporter assay.
CONCLUSIONS: miR-154 plays a prominent role in CaP proliferation by suppressing CCND2, and it may provide a new approach to the treatment of CaP.
Written by:
Zhu C, Shao P, Bao M, Li P, Zhou H, Cai H, Cao Q, Tao L, Meng X, Ju X, Qin C, Li J, Yin C. Are you the author?
State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Reference: Urol Oncol. 2013 Feb 18. pii: S1078-1439(12)00406-1.
doi: 10.1016/j.urolonc.2012.11.013
PubMed Abstract
PMID: 23428540
UroToday.com Investigative Urology Section
