OBJECTIVE: Our objective was to evaluate the levels of miR-210 in tumor and serum samples of conventional renal cell cancer (cRCC) patients to explore whether circulating miR-210 in serum can be used as a biomarker for the detection of cRCC.
METHODS: The paired samples from primary cRCC tumors and adjacent non-tumoral renal parenchyma were collected from 32 patients with cRCC. Serum samples were obtained from 68 patients with a cRCC before surgery, 10 samples after one week of surgery, and 42 healthy individuals were included in this study. Real-time PCR was used to measure the microRNA level. The expression of miRNAs was normalized using the dCT method. Expression levels of miR-210 were compared using the Mann-Whitney U test or Wilcoxon test. Diagnostic performance of serum miR-210 level was calculated by using the receiver operating characteristic (ROC) curve.
RESULTS: The average miR-210 level was higher in primary cRCC tissues than in normal tissue (p=0.004). For serum samples, the average level of miR-210 was significantly higher in cRCC patients than in controls (p<0.001). The serum miR-210 level yielded an AUC (the areas under the ROC curve) of 0.874 with a sensitivity of 81.0% and a specificity of 79.4%. Furthermore, the average serum level of miR-210 was significantly decreased in the patients one week after the operation (p=0.001).
CONCLUSION: Serum mi-210 may have a potential as a novel noninvasive biomarker for the detection of cRCC.
Written by:
Zhao A, Li G, Péoc'h M, Genin C, Gigante M. Are you the author?
Clinical Immunology Laboratory, North Hospital, CHU of Saint-Etienne, University of Jean-Monnet, Saint-Etienne, France
Reference: Exp Mol Pathol. 2013 Feb;94(1):115-20
doi: 10.1016/j.yexmp.2012.10.005
PubMed Abstract
PMID: 23064048
