OBJECTIVE: To investigate the expression and possible roles of Twist1 and Y-box-binding protein-1 (YB-1) in bladder cancer tissue.
Twist1 belongs to the family of basic helix-loop-helix transcription factors. A functional link between Twist1 and YB-1 has recently been determined to play an important role in bladder cancer cell lines.
MATERIALS AND METHODS: Frozen samples from 75 patients with bladder cancer were analyzed by quantitative real-time polymerase chain reaction (PCR). Formalin-fixed and paraffin-embedded tissues from 53 patients with bladder cancer were examined by immunohistochemistry.
RESULTS: Twist1 transcript levels were positively correlated with YB-1 transcript levels (coefficient of correlation = 0.42, P< 0.001), tumor grade (low grade vs. high grade; P< 0.001), invasiveness (non-muscle-invasive bladder cancer vs. muscle invasive bladder cancer; P = 0.0018), and metastasis (meta- vs. meta+; P< 0.001). YB-1 transcript level was also correlated with grade (P = 0.029) and invasiveness (P = 0.006). By immunohistochemistry, Twist1 expression was also correlated with YB-1 expression (P< 0.001). Further, both Twist1 and YB-1 expression were positively correlated with invasiveness (P = 0.007 and P = 0.002, respectively). Patients with high Twist1 expression and high YB-1 expression had lower overall survival rates, compared with patients with low expression (log-rank test, P = 0.040 and P<0.001, respectively).
CONCLUSIONS: These results suggest a functional link between Twist1 and YB-1, and they indicate that Twist1 and YB-1 promote bladder cancer progression.
Written by:
Song YH, Shiota M, Yokomizo A, Uchiumi T, Kiyoshima K, Kuroiwa K, Oda Y, Naito S. Are you the author?
Department of Urology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Reference: Urol Oncol. 2013 Feb 6. pii: S1078-1439(12)00396-1.
doi: 10.1016/j.urolonc.2012.11.003
PubMed Abstract
PMID: 23395237
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