BACKGROUND: An increasing body of evidence indicates that microRNAs play critical roles in androgen-independent prostate cancer (AIPC) growth.
However, the regulation of the expression of microRNAs in AIPC is not very clear. In this study, we investigated the role that the interaction between miR-200b-3p and p73 plays in the proliferation of AIPC.
METHODS: We compared several relevant microRNAs and cancer related genes between the androgen-dependent prostate cancer (ADPC) cell line and the AIPC cell line using quantitative real-time PCR (Q-PCR) and Western blot. Then we examined the effect of p73 and miR-200b-3p on the proliferation of AIPC and ADPC using CCK-8. Furthermore we investigated the regulation of miR-200b-3p by p73.
RESULTS: p73 and miR-200b-3p were both downregulated in the PC3 cell line (AIPC). Down-regulation of both p73 and miR-200b-3p increased the proliferation of ADPC cells cultured with androgen-free medium, while up-regulation of p73 and miR-200b-3p decreased the proliferation of AIPC cells. When p73 was over-expressed in the AIPC cell subline, miR-200b-3p expression increased accordingly, while p73 was inhibited in ADPC cells cultured with androgen-free medium and miR-200b-3p expression decreased significantly.
CONCLUSION: miR-200b-3p is down-regulated by low expression of p73 in AIPC cells, and this interaction contributes to the proliferation of AIPC.
Written by:
He M, Liu Y, Deng X, Qi S, Sun X, Liu G, Liu Y, Liu Y, Zhao M. Are you the author?
Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Reference: Prostate. 2013 Feb 6. Epub ahead of print.
doi: 10.1002/pros.22652
PubMed Abstract
PMID: 23389960
UroToday.com Investigative Urology Section
