It has been shown that human prostate cancer (PCa) cells induced apoptotic death of the most potent antigen-presenting cells, dendritic cells (DCs), which are responsible for the induction of specific antitumor immune responses.
Here, we investigated the function of 4-1BB on protecting DCs from prostate cancer-induced apoptosis with an agonistic mAb to 4-1BB. RM-1 cells and DCs were co-incubated for 48 h and DC apoptosis was assessed by Annexin Vassay. TNF-α and IL-12 production were assessed by enzyme-linked immunosorbent assay (ELISA) and Bcl-2 and Bcl-xL on DCs were analyzed by Western blot. We have shown that co-incubation of RM-1 cells with DCs is accompanied by an increased level of DCs apoptosis. Triggering 4-1BB on DCs resulted in increased resistance of DCs to RM-1 cells-induced apoptosis, which was owing to the up-regulated expression of Bcl-2 and Bcl-xL, and increased secretion of TNF-αand IL-12. These results demonstrate that triggering 4-1BB on DCs could increased resistance of DCs to PCa-induced apoptosis.
Written by:
Youlin K, Jianwei Z, Xin G, Li Z, Xiaodong W, Xiuheng L, Hengchen Z, Zhiyuan C. Are you the author?
Department of Urology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
Reference: Pathol Oncol Res. 2012 Oct 16. Epub ahead of print.
doi: 10.1007/s12253-012-9566-0
PubMed Abstract
PMID: 23070584
UroToday.com Investigative Urology Section
