AIM: We sought to investigate protein biomarkers for stress urinary incontinence (SUI) in vaginal tissues using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and examine if this is a reliable methodology to examine proteins in small tissue specimens.
MATERIAL AND METHODS: We compared protein expression profile of vaginal tissue from women with SUI and continent controls. A 22.6kDa peak was identified by subsequent weak cation-exchange, reverse-phase fractionation, gel electrophoresis, and trypsin digestion, then analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) and MALDI MS-MS. Biomarker identity and expression level were confirmed by Western-blotting and immunohistochemistry.
RESULTS: Expression of the 22.6kDa protein, identified as SM-22α, was significantly higher in women with SUI versus controls. A 3×3-mm tissue sample was sufficient for identification. Western-blot/immunohistochemistry confirmed the SELDI-TOS MS findings.
CONCLUSION: SM-22α, a marker for myofibroblasts, was identified as a biomarker of SUI. Differential protein profiling by SELDI-TOF MS is a powerful and reliable tool for urogynecological research as it allows us to study an array of proteins simultaneously using small tissue samples.
Written by:
Wen Y, Whitin J, Yu T, Cohen H, Polan ML, Chen B. Are you the author?
Department of Obstetrics and Gynecology Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Reference: J Obstet Gynaecol Res. 2012 Jan;38(1):89-96.
doi: 10.1111/j.1447-0756.2011.01690.x. Epub 2011 Dec 5.
PubMed Abstract
PMID: 22136672
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