OBJECTIVE: To investigate hydrogen sulfide and its synthases, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), in human prostatic tissue and cells.
METHODS: CBS and CSE in human prostatic tissue and cells were located using immunostaining. Western blot and a sulfur-sensitive electrode were used to evaluate the expression levels and catalytic activity of CBS and CSE. We analyzed the association between dihydrotestosterone-added or hormone-reduced medium-induced CBS/CSE protein levels with androgen receptor levels in prostate cancer lines. All experiments were repeated ≥3 times.
RESULTS: Endogenous hydrogen sulfide and its synthases existed in various areas of human prostatic tissue and cells. Cell activity and CBS/CSE protein levels were greatest in the androgen-dependent prostate cancer cell LNCaP among all cells and downregulated by dihydrotestosterone.
CONCLUSION: Hydrogen sulfide and its synthases in human prostatic tissue and cells were modulated by dihydrotestosterone, which could suggest a potential therapy for prostatic disease.
Written by:
Guo H, Gai JW, Wang Y, Jin HF, Du JB, Jin J. Are you the author?
Department of Urology, Peking University First Hospital, and Institute of Urology, Peking University, Beijing, China.
Reference: Urology. 2012 Feb;79(2):483.e1-5.
PubMed Abstract
PMID: 22310774
UroToday.com Investigational Urology Section
