MATERIALS AND METHODS: Cells were incubated with either vehicle (control), 1,25D or ZK. MMP-2 and MMP-9 activity was determined by gelatin zymography, while ICAM-1 levels were assessed by Western blot analysis and immunocytochemistry.
RESULTS: Compared to the controls, 1,25D and ZK caused a marked dose-dependent decrease in the gelatinolytic activity of the MMPs under study, particularly when ZK was used. Likewise, ICAM-1 was down-regulated in the cells incubated with 1,25D or ZK.
CONCLUSION: Vitamin D analogs appear to be involved in the regulation of extracellular MMP activity and membrane adhesion molecule expression. Further studies, both in vitro and in vivo, are needed to define their role as potential therapeutic tools.
Written by:
Stio M, Martinesi M, Simoni A, Zuegel U, Steinmeyer A, Santi R, Treves C, Nesi G. Are you the author?
Department of Biochemical Sciences, University of Florence, Florence, Italy.
Reference: Anticancer Res. 2011 Dec;31(12):4091-8.
PubMed Abstract
PMID: 22199266
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