Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.
Systems biology in reproductive medicine. 2016 Apr 22 [Epub ahead of print]
Raheel Samuel, Odgerel Badamjav, Kristin E Murphy, Darshan P Patel, Jiyoung Son, Bruce K Gale, Douglas T Carrell, James M Hotaling
a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA., a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA., c Huntsman Cancer Institute, University of Utah , Salt Lake City , Utah , USA., d Division of Urology, Department of Surgery , University of Utah , Salt Lake City , Utah , USA., e Department of Electrical & Computer Engineering , University of Utah , Salt Lake City , Utah , USA., b Department of Mechanical Engineering , University of Utah , Salt Lake City , Utah , USA., a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA., a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA.