OBJECTIVE: To identify the differential urinary metabolic pattern of oligozoospermic infertile men and to determine the potential biomarkers indicative of infertility.
DESIGN: Observational study.
SETTING: University hospital.
PATIENT(S): Totals of 158 fertile volunteers and 135 oligozoospermic infertile men.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Urinary metabolic profiles were acquired with the use of liquid chromatography/mass spectrometry. Potential oligozoospermic biomarkers were screened from orthogonal projections to latent structures discriminant analysis and further evaluated by receiver operating characteristic analysis. The Spearman correlations between the individual sets of biomarkers and between biomarkers and sperm parameters were investigated. The disrupted biologic pathways which the biomarkers were involved in also were analyzed.
RESULT(S): Oligozoospermic infertile men could be differentiated from fertile control subjects based on altered urinary metabolic profiles. A total of ten potential biomarkers were screened and tentatively identified. Among those, decreased acylcarnitines, aspartic acid, and leucylproline and increased adenine and methylxanthine were strongly associated with oligozoospermic risk. Many biomarkers were associated with sperm concentration and amplitude of lateral head displacement. The combined pattern of acetylcarnitine, carnitine C3:1, and aspartic acid provided moderate diagnostic power.
CONCLUSION(S): Urinary metabolomics identified unique metabolic pattern of oligozoospermic infertility. The potential biomarkers suggested that oligozoospermia may be tightly associated with energy consumption and antioxidant defenses in spermatogenesis.
Written by:
Zhang J, Huang Z, Chen M, Xia Y, Martin FL, Hang W, Shen H. Are you the author?
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, People's Republic of China; Department of Chemistry, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People's Republic of China; Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, People's Republic of China; Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster, United Kingdom.
Reference: Fertil Steril. 2014 Apr 17. pii: S0015-0282(14)00288-X.
doi: 10.1016/j.fertnstert.2014.03.033
PubMed Abstract
PMID: 24746742
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