Biological mechanisms contribute to the relationship between polycyclic aromatic hydrocarbon (PAH) exposure and infertility in males by altering semen quality.
The aim of the present study was to evaluate the impact of PAHs on male infertility using the sperm chromatin dispersion test (Halo sperm assay).
Sixty-six (66) infertile males under 45 years of age were examined for the determination of urinary metabolite and oxidative stress by measuring lipid peroxidation and antioxidant activity of glutathione and glutathione-s-transferase, as well as hormonal activity of follicle stimulating hormone (FSH), testosterone and prolactin and semen quality.
There was an increased level of urinary metabolite of 1-hydroxy pyrene, 1-hydroxy naphthalene and 2-hydroxy naphthalene in the urine of the infertile group. In addition, elevated concentrations of malondialdehyde coincided with a decreased level of antioxidants, leading to oxidative stress in the infertile group. Semen samples showed 30% sperm deoxyribonucleic acid (DNA) fragmentation.
The data provide strong evidence of a statistical threshold for semen samples containing 30% sperm DNA fragmentation resulting in a reduced level of pregnancy success.
Obtained.
Study approval was given by the ethics committee of Alexandria University (United States Department of Health and Human Services, institutional review board registration (IRB), IORG0008812 Medical Research Institute, expires 4/8/2019, OMB No: 0990-0279).
The authors declare no competing financial interests.
Journal of health & pollution. 2019 Mar 14*** epublish ***
Aziza A Saad, Tarek Hussein, Amany El-Sikaily, Mohamed A Abdel-Mohsen, El-Hassan Mokhamer, Amany I Youssef, Jihan Mohammed
Applied Medical Chemistry, Medical Research Institute, Alexandria University, Alexandria, Egypt., Dermatology, Venereology and Andrology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt., Marine Pollution Department, Marine Environment Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt., Molecular Biology lecturer, Zoology Department, Damnhour University, Damanhur, Egypt.