OBJECTIVE - Nitric oxide (NO) has recently gained increasing recognition as an important neurotransmitter of functions in the lower urinary tract. This prospective study firstly investigated the association of eNOS G894T gene polymorphism with the responsiveness to α1-blocker in men with benign prostatic hyperplasia related lower urinary tract symptoms (BPH/LUTS).
MATERIALS AND METHODS - 136 patients with BPH/LUTS were recruited from urology outpatient clinics in a university hospital. Oral therapy with doxazosin- GITS 4 mg once-daily was given for 12 weeks. The drug efficacy was assessed by the changes from baseline in the total International Prostate Symptom Score (IPSS), maximum urinary flow rate (Qmax) and post-void residual urine volume (PVR) at 12 weeks of treatment. The responders to doxazosin GITS were defined as those who had a total IPSS decrease of more than 4 points from baseline. The eNOS G894T polymorphisms were determined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method RESULTS: Patients had statistically significant improvements in total IPSS, QoL and Qmax (P<0.01) for a 12-week period of treatment. Using multiple logistic regression analysis adjusted for age and IPSS, our results showed that eNOS 894T allele carrier was an independent factor for drug non-responder (p=0.03, OR=4.19). Besides, the decreased responder rate (p=0.01), as well as the lower improvements of IPSS (p=0.02) and Qmax (p=0.03), were significantly associated with an increment of the T allele number.
CONCLUSIONS - The presence of eNOS 894T allele had a significantly negative impact on responsiveness to α1-blocker in BPH/LUTS treatment, suggesting that eNOS G894T gene polymorphisms may play an implication as a genetic susceptibility factor for drug efficacy in men with BPH/LUTS. This article is protected by copyright. All rights reserved.
BJU international. 2016 Mar 04 [Epub ahead of print]
Yung-Chin Lee, Yung-Shun Juan, Chia-Chu Liu, Bo-Ying Bao, Chii-Jye Wang, Wen-Jeng Wu, Chun-Nung Huang, Shu-Pin Huang
Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Department of Pharmacy, China Medical University, Taichung, Taiwan., Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.