These authors tried to quantify formation and progression of biofilm in urinary catheters of different duration dwell times. 74 catheters of different dwell times were collected from patients at the Duke University Medical Center. These catheters were analyzed for biofilm formation.
5 cm portions from the middle of the catheter was longitudinally split into equal halves, internal lumens were stained with crystal violet and percent of urinary coverage was quantified. External biofilm around the catheter was stained with a crystal violet and percent of urinary coverage was quantified. Results showed that biofilm developed as early as 13 hours after catheter placement with 13.8% of catheter coverage. The biofilm thickness was also noted. The biofilm on the outside of the catheter was less than internal biofilm, statistically significant. The authors concluded that this is the first human study documenting the natural history of biofilm in clinical practice. Biofilm formation starts immediately after catheter implantation with 50% biofilm coverage as early as day 1, reaching 93% by day 28. The majority of the biofilm was found within the catheter suggesting a very specific CAUTI and biofilm location.
Presented by: Ibrahim, et al. at 2016 AUA Annual Meeting - May 6 - 10, 2016 – San Diego, California, USA
Written by:
Diane K. Newman, DNP, Adjunct Professor of Urology in Surgery, Research Investigator Senior and Co-Director, Penn Center for Continence and Pelvic Health
University of Pennsylvania, Division of Urology, 3400 Spruce Street, 3rd Floor Perelman Bldg, Philadelphia, PA. 19104
Presented by: Ibrahim, et al. at 2016 AUA Annual Meeting - May 6 - 10, 2016 – San Diego, California, USA
Written by:
Diane K. Newman, DNP, Adjunct Professor of Urology in Surgery, Research Investigator Senior and Co-Director, Penn Center for Continence and Pelvic Health
University of Pennsylvania, Division of Urology, 3400 Spruce Street, 3rd Floor Perelman Bldg, Philadelphia, PA. 19104