Department of Mechanical Engineering and Department of Urology, Stanford University, California 94305, United States.
We present a novel assay for rapid detection and identification of bacterial urinary tract infections using isotachophoresis (ITP) and molecular beacons. We applied on-chip ITP to extract and focus 16S rRNA directly from bacterial lysate and used molecular beacons to achieve detection of bacteria specific sequences. We demonstrated detection of E. coli in bacteria cultures as well as in patient urine samples in the clinically relevant range 1E6-1E8 cfu/mL. For bacterial cultures we further demonstrate quantification in this range. The assay requires minimal sample preparation (a single centrifugation and dilution), and can be completed, from beginning of lysing to detection, in under 15 min. We believe that the principles presented here can be used for design of other rapid diagnostics or detection methods for pathogenic diseases.
Written by:
Bercovici M, Kaigala GV, Mach KE, Han CM, Liao JC, Santiago JG. Are you the author?
Reference: Anal Chem. 2011 Jun 1;83(11):4110-7.
doi: 10.1021/ac200253x
PubMed Abstract
PMID: 21545089
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